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Detailed characterizations of picosecond laser electronic excitation tagging (PLEET) in pure nitrogen ( ${\mathrm{N}}_2$) and air with a 24 ps burst-mode laser system have been conducted. The burst-mode laser system is seeded with a 200 fs broadband seeding laser to achieve short pulse duration. As a non-intrusive molecular tagging velocimetry (MTV) technique, PLEET achieves “writing” via photo-dissociating nitrogen molecules and “tracking” by imaging the molecular nitrogen emissions. Key characteristics and performance of utilization of a 24 ps pulse-burst laser for MTV were obtained, including lifetime of the nitrogen emissions, power dependence, pressure dependence, and local flow heating by the laser pulses. Based on the experimental results and physical mechanisms of PLEET, 24 ps PLEET can produce similar 100 kHz molecular nitrogen emissions by photodissociation, while generating less flow disturbance by reducing laser joule heating than 100 ps PLEET.